The PF-Tek was developed and first made public in by www. This tek made the cultivation of mushrooms at home feasible for complete beginners utilizing commonly available materials. The growing method I describe here is based on the PF-tek but includes a few modifications that are time tested, and are in my opinion superior to the original PF-tek. I strongly recommend you to read the original PF-Tekif you haven't done so already, to see a different approach to some steps.
The PF-Tek for Simple Minds is as simple and as foolproof as it gets, but is not completely foolproof of course. Following it will give you good chances of succeeding and a good idea on the general process and time line and prepare you for higher yielding teks, like using whole grains and dung. The substrate is mixed, filled in jars, sterilized and inoculated with mushroom spores.
After the substrate is fully colonized the substrate cakes fruit in a humid container. By growing mushrooms indoors on a nutritious substrate you create conditions than not only favor the growth of the mushrooms, but also the growth of a large number of other organisms molds, bacteriamany of them potentially hazardous to the health. To ensure that only the desired mushroom is grown, it is very important to assure cleanliness in all of the cultivation related procedures.
Before you work, wash your hands with antibacterial soap and warm water. Afterwards, wipe them dry and rub with Lysol or isopropyl alcohol iso-propanol. Keep the place where you do the inoculation and fruiting dust free and clean and don't bring in dirty clothing or shoes.
Personal hygiene is equally important. Dirty hands and even dirty hair are a hotbed for all kinds of unwanted microorganisms which can destroy your cultivation project. Vermiculite is made from a naturally occurring mineral - mica.
Crushed mica containing water is heated and expands to a volume several times greater than that of the untreated mica. Vermiculite is able to hold several times its own weight in water and it gives the substrate an airy structure.
Vermiculite is available in several grades, the middle and the middle-fine grade are most suitable for cultivation purposes. Canada www. United Kingdom www. Australia users. Generally you can get vermiculite in garden and hydroponic stores, in some regions also in pet shops.
BRF is available in health food shops either already ground, sometimes though there is only whole brown rice available.
In this case you can grind the rice either in the shop or if this option is not available, grind your own using an electric coffee grinder. BRF is also available at Ebay: BRF is best kept cool and dry for prolonged periods of time, since it can easily become rancid because of the fat content of its husk.
If you are unable to find BRF you can also use whole rye flour, ground millet or ground millet based birdseed with similar results. Water used for the substrate preparation should have drinking water quality. Tap water is usually OK, but if you're not sure about it, better use bottled drinking water or mineral water. A plastic syringe with needle attached containing 10cccc suspension of mushroom spores in water. The color of the suspension varies from completely translucent to slightly violet depending on the quantity of the spores in the solution.
Spores are microscopic so as long as you see at least a few specs in more or less clear water the syringe should have plenty of spores.
Available through the internet, for instance www. Bigger jars take much longer to colonize and are not recommended. Kerr wide mouth mason jar half pint, 8oz.Spore syringe. A spore syringe will contain the mushroom spores needed to inoculate the substrate and begin growing. Vermiculite is a porous, inert substance that we will use to maintain moisture content inside the cases and provide space for the mycelium to grow.
The fine ground vermiculite is preferable, but course vermiculite will also work. Brown rice flour. Commonly referred to as by its acronym, BRF, brown rice flour is a hyper-nutritious substrate that will provide nutrients to our mushroom.
Mason jars. We will use the a flame to sterilize the spore syringe 's needle between the inoculations of each jars. A butane lights, gas stove or alcohol lamps will all work just fine. The substrate is made of vermiculite, brown rice flourand purified water. You'll want to mix the vermiculite and water first.
Make sure that the vermiculite has soaked up all of the water it can before adding the brown rice. Then add the brown rice flour to the hydrated vermiculite.
The flour should stick to the outside surface of the vermiculite. There should be no water in the bottom of your mixing bowl after thoroughly stirring. Fill the jars up to mm about half-inch below the rim.
Do not compress the mixture into the jars. We want there to be air spaces through which the mushroom mycelium will be able to grow. Wipe the top of the jars clean inside and out with a clean paper towel in order to ensure there is no substrate sticking on the edges. We want nothing nutritious to be left behind. Fill the remaining space inside the jar with dry vermiculite. This will serve as a contamination barrier. C lose with the lids.
Growing Mushrooms: PF Tek
The lids should have 4 holes near the edges and cover the holes with masking-tape or micropore tape. The holes will be used to inoculate the jars with spore siringe. Cover the jars with aluminium foil so that condensation in the pressure cooker won't drip on the lids and through the holes.
That might cause too much water to enter the jars. With brown rice flour as our substrate, we have two options for sterilization, a pressure cooker or steaming. This will avoid direct contact of the glass jars with the bottom of the cooking pot, which might otherwise cause the jars to crack.
This method works with brown rice flour because it sterilizes more easily at lower temperatures than other spawn substrates. Boil ml half-pint to a pint jars for minutes.Please login or register. Pages: [ 1 ] 2. Author Topic: Average time for cakes to fruit? Read times crazyd Full Member Offline Posts: Hey guys. I've had my dunked and rolled cakes in my fruiting chamber for 4 days now. I built a chamber using an aquarium air pump that is pushing air through 2 rock bublers, up through water and clay pellets.
It looks to be very humid inside and I have kept them in light about 10 hours a day, just in my bathroom, getting light from 2 normal bulbs. Average temp has probably been 73 or so. The cakes very quickly showed new mycelial growth. What is a normal timeframe to start to see actual fruiting? These are mazatapecs Thanks! Probably within the week, cut ur light down to Is there enough FAE? What is FAE? Fresh Air Exchange. We're not always doing business, but we're always open.
Gcrazy Newbie Offline Posts: 4. Yeah, mine have been in there 1 week as of tonight. No fruiting yet. I'm still not sure what an average time might be? Seems I read that several days is normal? But I also think I saw that sometimes it can take weeks. Anyone know?How to make a SGFC for at home mushroom cultivation
MT Sr. Member Offline Posts: My cakes usually start to pin about five to six days after the dunk and roll. Lol, back in the day when I was doing pf-tek. I had puerto rican's take around a month before they started pinning, but once they pinned they were full grown and potent as all hell in a couple days. I've definitely got ONE pin on one of my cakes.
I guess that is a good sign Okay, so my one cake with the one pin is doing okay, but now I've got another with a bunch of small pins!
This is very exciting! It has been 9 days since I placed them in the fruiting chamber. The 2 with pins birthed 12 days ago. The spiky mycelium one was birthed 5 days ago BTW what strain.Did you use this instructable in your classroom? Add a Teacher Note to share how you incorporated it into your lesson. To begin growing your own mushrooms you will need the following for the PF tek: - Pressure cooker Check thrift stores such as savers and goodwill, also browse your local craigslist.
Be sure to use wide mouthed tapered jars! Spore syringes are the easiest way to get started growing mushrooms with this technique. They can be ordered online from a variety of sources. To prepare your jars, you will need a hammer and nail. Remove the jar lid and lay the flat lid rubber side up on a table. Take a nail and make 2 - 4 holes evenly spaced around the edge.
The sharp edge around the hole can tear the foil in the next few steps. The substrate is what the fungus will feed off of. It will consist of brown rice flour, vermiculite and water. First, take the dry ingredients and mix them in a bowl. After they are well mixed add the water.
I have heard of adding 'worm castings' earthworm poop to substrate to give better flushes of mushrooms. Once your substrate is well mixed and moist, begin filling your prepared jars with the substrate. Don't pack the mixture down, just loosely fill the jar. I just filled my jars up to the threading for the lid.
Fill the rest of the jar with dry vermiculite. This is to make a barrier between the substrate and contaminants in the air. Now close up all your jars, with the rubberside facing upwards upside down from how they were designed Place a square of foil over the lid covering the holes and sealing the jar from contaminants in the air.
Crumple it down nice and tight, forming it around the jar lid. Put about 3 inches of water in your pressure cooker and place as many jars as you can, stacking them if you have to. I had no problem putting jars directly on the bottom of the cooker, but I have seen people put old canning jar rings in the bottom to help prevent jars from cracking. READ your pressure cooker's manual if you have it!
It can be a dangerous tool if improperly used. Close up your cooker and put it on a stove. I used a BBQ side stove to keep heat out of the house.
PF-Tek for Simple Minds
This is around psi depending on the manufacturer. Let the jars and substrate sterilize for about an hour. Once the hour is up, let the cooker stand for about 3 hours to cool down, you can also sterilize in the evening and let it cool down overnight. Inoculation is the process of introducing spores of your chosing into the sterilized substrate to take hold and grow. In this step, be sure to take precautions to prevent contamination of the jars!!
Leave the jars in the pressure cooker until you are ready to Inoculate.We like to pick mushrooms. Unfortunately, mushrooms themselves are picky — and getting mushrooms to fruit properly requires that we present them with ideal growing conditions.
Building a Shotgun Fruiting Chamber commonly referred to as an SGFC is a quick and easy way to provide the perfect balance of fresh air and humidity that mushrooms demand. A shotgun fruiting chamber is nothing more than a good sized clear tote, covered in holes, and filled with a few inches of wet perlite in the bottom. The holes allow for fresh air and gas exchange, while the perlite serves as a way to maintain humidity. The tote should be clear in order to allow for an adequate amount of natural light to be passed through to your mushrooms.
Perlite is a natural volcanic mineral that, when expanded, forms a coarse and porous granule with a large relative surface area.
All the nooks and crannies in the perlite granules are able to hold water, which evaporates over time. This evaporation from the perlite is what allows for the high relative humidity in your fruiting chamber. A small pile of coarse perlite.
You should be able to find it at your local garden center. Once you get the materials you require, you should be able to build your SGFC in less than an hour. In order to get proper air flow through the SGFC, you want to have evenly spaced holes on all 6 sides of your tote- including the lid and the bottom.
Mark out all your holes with a sharpie. You will also need to ream out the holes and make sure there are no shards of plastic stuck on the edge. Fill the bottom of your SGFC with a couple inches of perlite. You also want to make sure you use coarse perlite. If the grind is too fine, you will have a lot of perlite coming through the holes you drilled, making a mess of things. Soak the perlite by pouring water into your SGFC and mixing it around until the perlite is evenly moist.
There is no need to soak it so much that water is pouring out the bottom of the tote. The intention is just to coat the perlite in water so that in can evaporate over time, increasing the humidity in your SGFC. You now have a reasonably humid environment able to exchange gasses and receive fresh air- perfectly adequate chamber for growing all sorts of mushrooms on a small scale. A typical use for a SGFC is to grow mushrooms using the PF technique, but you can just as easily use a shotgun terrarium with supplemented sawdust fruiting blocks, or even mushroom grow kits.
Although not entirely necessary, a hygrometer is a useful tool that can be placed in your fruiting chamber to help you monitor and manage the humidity levels.
It will give you a really good idea of whether or not you need to spray the inside of the chamber.Mushrooms do not use light in the same way that plants do for photosynthesis ; rather, light is a signal that tells the fungus to start its fruiting stage.
In nature, light would tell the fungus that it has reached the outside of a dung pile and that where the light hits it is a good place to create a fruit body. Most types of light source will work. Indirect sunlight and florescent bulbs temperature k both work. When using indirect sunlight, care must be taken to rotate the chamber so all sides get even amount of sunlight, or fruiting will occur more heavily on the sunlit side, thus reducing yield.
When using artificial light, a common approach to simulate day and night is to use a simple outlet timer on a 12 hours on and 12 hours off schedule.
PF-Tek for Simple Minds
Some cultivators prefer to leave their lights on for 24 hours a day and find that this produces satisfactory results. There is debate as to whether any darkness is needed at all for successful fruiting, however it is not debated that at least 1 or 2 hours of light is needed per day. The sides of the fruiting chamber should have some method of blocking light from the bottom of the substrate to about three inches above it on the side of the chamber.
If this is not done, side pinning and bottom pinning will occur. This is undesirable as it can produce a fruit body that is stunted, as well as bottom and side fruit are often not seen, and allowed to mature, die and rot on the substrate allowing a potent vector for contamination. Lower levels of CO2 are also a signal for the mycelium to create fruit bodies.
Again, in nature when the mycelium reaches the outside of the dung pile, there is much more fresh air than inside the pile. The more clean fresh air you can get into your fruiting chamber the better, so long as you are able to keep the proper level of humidity. Also, some contaminants thrive in stale air with high CO2 levels, so FAE is also important for avoiding conditions favorable to some molds and bacteria.
Depending on the type of fruiting chamber used, there are various ways to achieve optimum FAE. Some cultivators use a fish tank air pump on a digital timer to supply fresh air every other hour or so. Others simply open up the lid and fan with a magazine, but in a dirty grow room, this approach would increase risk of contamination. Some fruiting chambers are outfitted with cool mist or sonic humidifiers in order to provide both FAE and higher relative humidity.
The humidity with a fruiting chamber must remain high in order to encourage pinning and to all the growing mycelium to retain as much water as it can hold. A hygrometer can be used to measure RH, but many experienced cultivators can gauge relative humidity by examining water condensation on the walls of the fruiting chamber.
Relative humidity is carefully controlled when a fruiting chamber is used. The mycelium itself can create and maintain its own humidity relatively well, especially once it has fully colonized and fruit bodies are starting to grow. However, there are many different techniques which can be used to aid in maintaining proper humidity. Misting with a spray bottle is not a humidification technique in itself so much as a supplement to other primary techniques.
Misting is commonly used in order to replenish humidity after fanning for fresh air exchange or to replenish the moisture in casing layers. Mycelium and fruit bodies should never be directly sprayed with water.By Guest shroomnoob, March 12, in Mycology.
I someone lived in a country where growing psilocybin was legal and wanted to use some once per month, would the following tek be beter than messing around with humidity levels and perlite in a fruiting chamber? I didn't realize that mycelium contained psilocybin. Doing it this way obviously produces less yeilds than 3 or 4 flushed of fruit but if you only wanted 12 hits per year then you would only need to produce 3 jars at a time, 4 times a year.
What are potential drawbacks to this method? Is there a risk of drinking contaminants that are in the jar? Thanks for any info. Yeah that's pretty much the perfect tek for a infrequent user. Realistically, the mycelial cake is probably equal avg potency to the fruitbodies, considering they consist of the same thing. Probably best to follow the tek and put the jars into the freezer after the two weeks of full colonisation.
Hyphal, I'd say, why bother fruiting a jar if one jar a month is all you need? Less work, more stealth, no worries about wintertime fruiting temps, etc etc. If you wish to further the genetic line via spores you can slice off a small disc of the cake to provide a few fruits for prints before putting the rest in the freezer where it will neither contam or degrade.
Crystals precipitated in proof "Everclear" left beaker extract from 16 grams dried shrooms right tube extract from 9 grams dried fungi. Place the flask in a hot water bath for four hours. Filter the liquids with suction through a filter paper in a buchner funnel with Celite to prevent clogging. Collect and save the filtrate liquids.
Heat the slurry the mush in the filter paper two more times in methanol as before, filter, and accumulate the liquids of the three extractions. To be certain that all of the alkaloids have been extracted do a small extraction with a portion of the used slurry and test with Keller's reagent glacial acetic acid, ferrous chloride, and concentrated sulfuric acid.
If there is a violet indication, alkaloids are still present and further extraction is in order. In an open beaker evaporate the liquids to total dryness with a hot water bath or by applying a hair dryer.
Be certain that all traces of methanol have been removed. Greater purification can be achieved, but would require other solvents and chromatography equipment and is hardly necessary.
Each grams of dried mycelium should yield about 2 grams of extracted material. Theoretically psilocin should have the same effect upon the user as psilocybin. The only difference between the two is that the later has a phosphate bond which disappears immediately after assimilation in the body. In other words, in the body psilocybin turns into psilocin.
Psilocybin is a fairly stable compound, but psilocin is very susceptible to oxidization. It is best to keep the extracted material in a dry air tight container under refrigeration. A sack of silica-gel can be placed in the container to capture any moisture that may enter. We will figure the average dose as 10 mg. The crude alkaloid extraction process given here yields a brownish crystalline powder that is at least 25 percent pure.
Each mason jar should contain at least 50 grams of wet mycelium.